Methoden & Techniken

Dieser Bereich bietet ein tieferes Verständis und Troubleshooting für ausgewählte wissenschaftliche Methoden.


14.10.2019

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The protocol described below is the Atlas Antibodies

standard immunohistochemistry protocol optimized

for Triple A Polyclonals and PrecisA Monoclonals

 

Deparaffinization

Paraffin sections of 4 µm thickness are baked overnight at 50°C. Prior to immunostaining, deparaffinization and hydration are performed in xylene and graded ethanol to distilled water. During hydration, we perform 5 minutes blocking for endogenous peroxidase in 0.3% H2O2 in 95% ethanol. Read More 

 


12.09.2019

How to Choose Membrane Protein Isolation Kits


Plasma membrane (PM) proteins play a critical role in a variety of physiological and pathological processes.  Signal transduction, molecular transport and cell-cell interactions are all mediated by PM proteinsPM proteins include a variety of important proteins such as neurotransmitter receptors, G-proteins... Read More 

 


 

13.09.2019

In vivo magnetofection

 

The main problems currently associated with systemic gene vector administration (gene therapy) include biodistribution of gene vector throughout the body, the lack of specificity towards a pathological site (bioavailability at the target site), the necessity of a large dose to achieve high local concentration, non-specific toxicity, inactivation of vectors due to undesired interactions with components of the in vivo milieu and other side effects due to high vector doses. Magnetofection™ resolves the problems... Read More

 


 

13.09.2019

SpheroTechnical Notes #8 - Calibration and Performance Tracking of Flow Cytometer Using SPHERO™ Calibration Particles

 

The SPHERO™ Calibration Particles are versatile, stable, economical and convenient to use. These particles contain a mixture of fluorochrome which are spectrally similar to many of the fluorochromes used in flow cytometry. As a result, they are used for routine alignment, day-to-day performance verification and long term performance tracking of several channels of flow cytometers in one run. These particles are very stable... Read More

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