Methoden & Techniken
Dieser Bereich bietet ein tieferes Verständis und Troubleshooting für ausgewählte wissenschaftliche Methoden.
Immunohistochemistry (IHC) Protocol using Proteinase K Based Antigen Retrieval
Immunohistochemistry protocol for formalin-fixed, paraffin embedded tissues using Proteinase K based antigen retrieval. It describes sample preparation as well as the immunostaining procedure with different recommended detection systems.
Standard Immunohistochemistry (IHC) Protocol
The protocol described is the Atlas Antibodies standard immunohistochemistry protocol optimized for Triple A Polyclonals and PrecisA Monoclonals. It includes sample preparation, staining procedure and recommended reagents.
How to Choose Membrane Protein Isolation Kits
Plasma membrane (PM) proteins play a critical role in a variety of physiological and pathological processes. Signal transduction, molecular transport and cell-cell interactions are all mediated by PM proteins. PM proteins include a variety of important proteins such as neurotransmitter receptors, G-proteins i.a.
In vivo magnetofection
The main problems currently associated with systemic gene vector administration (gene therapy) include biodistribution of gene vector throughout the body, the lack of specificity towards a pathological site (bioavailability at the target site), the necessity of a large dose to achieve high local concentration, non-specific toxicity, inactivation of vectors due to undesired interactions with components of the in vivo milieu and other side effects due to high vector doses. Magnetofection™ resolves these problems.
SpheroTechnical Notes #8 - Calibration and Performance Tracking of Flow Cytometer Using SPHERO™ Calibration Particles
The SPHERO™ Calibration Particles are versatile, stable, economical and convenient to use. These particles contain a mixture of fluorochrome which are spectrally similar to many of the fluorochromes used in flow cytometry. As a result, they are used for routine alignment, day-to-day performance verification and long term performance tracking of several channels of flow cytometers in one run.
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