Methoden & Techniken
Dieser Bereich bietet ein tieferes Verständis und Troubleshooting für ausgewählte wissenschaftliche Methoden.
The protocol described below is the Atlas Antibodies
standard immunohistochemistry protocol optimized
for Triple A Polyclonals and PrecisA Monoclonals
Paraffin sections of 4 µm thickness are baked overnight at 50°C. Prior to immunostaining, deparaffinization and hydration are performed in xylene and graded ethanol to distilled water. During hydration, we perform 5 minutes blocking for endogenous peroxidase in 0.3% H2O2 in 95% ethanol. Read More
How to Choose Membrane Protein Isolation Kits
Plasma membrane (PM) proteins play a critical role in a variety of physiological and pathological processes. Signal transduction, molecular transport and cell-cell interactions are all mediated by PM proteins. PM proteins include a variety of important proteins such as neurotransmitter receptors, G-proteins... Read More
In vivo magnetofection
The main problems currently associated with systemic gene vector administration (gene therapy) include biodistribution of gene vector throughout the body, the lack of specificity towards a pathological site (bioavailability at the target site), the necessity of a large dose to achieve high local concentration, non-specific toxicity, inactivation of vectors due to undesired interactions with components of the in vivo milieu and other side effects due to high vector doses. Magnetofection™ resolves the problems... Read More
SpheroTechnical Notes #8 - Calibration and Performance Tracking of Flow Cytometer Using SPHERO™ Calibration Particles
The SPHERO™ Calibration Particles are versatile, stable, economical and convenient to use. These particles contain a mixture of fluorochrome which are spectrally similar to many of the fluorochromes used in flow cytometry. As a result, they are used for routine alignment, day-to-day performance verification and long term performance tracking of several channels of flow cytometers in one run. These particles are very stable... Read More
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