Human Foreskin Fibroblast Whole Cell Lysate, Unconjugated

Produktübersicht

• Artikelname: Human Foreskin Fibroblast Whole Cell Lysate, Unconjugated
• Artikelnummer: BYT-ORB348677
• Hersteller Artikelnummer: orb348677
• Alternativnummer: BYT-ORB348677-500
• Hersteller: Biorbyt
• Kategorie: Sonstiges
• Applikation: SDS-PAGE, WB
• Konjugation: Unconjugated
• Alternative Synonym: Human Foreskin Fibroblast Whole Cell Lysate, Human Foreskin Fibroblast WCL, HFF Cell Lysate, foreskin lysate

Produktbeschreibung

Human Foreskin Fibroblast Whole Cell Lysate

Produkteigenschaften

• Konzentration: 1.0 mg/ml
• Puffer: Preservative: None. Stabilizer: 10% (v/v) Glycerol. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
• Quelle: Human
• Reinheit: The cells were grown in Dulbeccos medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodiu
• Formulierung: Liquid (sterile filtered)

Anwendungsbeschreibung

• Application Verdünnung: WB: User Optimized
• Anwendungsbeschreibung: ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been