Recombinant Rat TNF alpha

Produktübersicht

• Artikelname: Recombinant Rat TNF alpha
• Artikelnummer: EBT-EPT089
• Hersteller Artikelnummer: EPT089
• Alternativnummer: EBT-EPT089-50
• Hersteller: ELK Biotechnology
• Kategorie: Proteine/Peptide
• Alternative Synonym: Tumor Necrosis Factor, Cachectin, TNF-Alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, Tumor Necrosis Factor, Membrane Form, Tumor Necrosis Factor, Soluble Form, Tnf, Tnfa, Tnfsf2

Produktbeschreibung

Recombinant Rat Tumor Necrosis Factor is produced by our E.coli expression system and the target gene encoding Leu80-Leu235 is expressed.

Produkteigenschaften

• Molekulargewicht: Molecular weight: 17.4 KDa. Apparent molecular weight: 14 KDa, reducing conditions
• UniProt: P16599
• Reinheit: Greater than 95% as determined by reducing SDS-PAGE.

Anwendungsbeschreibung

• Anwendungsbeschreibung: Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100µg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.. Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. Background: Tumor necrosis factor alpha (TNF-alpha, TNFSF2) is the prototypic ligand of the TNF superfamily. Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism