AA3-DLin is an ionizable lipid used in generating lipid nanoparticles (LNPs) for RNA delivery. Unlike LNPs formulated with other ionizable lipids, AA3-DLin LNPs performed best with higher proportions of phospholipid and lower cholesterol (40:40:25:0.5 AA3-DLin/DOPE/cholesterol/DMG-PEG). AA3-DLin containing LNPs were able to deliver full-length SARS-COV2 spike protein mRNA to BALB/c mice and generate a strong immune response.
