HIF-1-alpha (9U11) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE12020
Produktübersicht
Artikelname:
HIF-1-alpha (9U11) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE12020
Hersteller Artikelnummer:
AMRe12020
Alternativnummer:
EKL-AMRE12020-50UL, EKL-AMRE12020-100UL, EKL-AMRE12020-200UL
Hersteller:
EnkiLife
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ICC, IP, WB
Spezies Reaktivität:
Human, Mouse, Rat
Konjugation:
Unconjugated
Alternative Synonym:
HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A
Produktbeschreibung
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5-[AG]CGTG-3 within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:11292861, PubMed: 11566883, PubMed: 15465032, PubMed: 16973622, PubMed: 17610843, PubMed: 18658046, PubMed: 20624928, PubMed: 22009797, PubMed: 9887100, PubMed: 30125331). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed: 11292861, PubMed: 11566883, PubMed: 15465032, PubMed: 16973622, PubMed: 17610843, PubMed: 20624928, PubMed: 22009797, PubMed: 9887100, PubMed: 30125331). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed: 22009797). Heterodimerizes with ARNT, heterodimer binds to core DNA sequence 5-TACGTG-3 within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300 (PubMed: 9887100, PubMed: 16543236). Activity is enhanced by interaction with NCOA1 and/or NCOA2 (PubMed: 10594042). Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP (PubMed: 10202154, PubMed:
Produkteigenschaften
Klonalität:
Monoclonal
Molekulargewicht:
93kDa
NCBI:
3091
UniProt:
Q16665
Puffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Reinheit:
Affinity purification
Formulierung:
Liquid
Target-Kategorie:
HIF1A
Anwendungsbeschreibung
Application Verdünnung:
WB 1:500-1:2000,ICC/IF 1:200-1:500,IP 1:20-1:50
