PKC beta 1 (9E18) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE16192
Produktübersicht
Artikelname:
PKC beta 1 (9E18) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE16192
Hersteller Artikelnummer:
AMRe16192
Alternativnummer:
EKL-AMRE16192-50UL, EKL-AMRE16192-100UL, EKL-AMRE16192-200UL
Hersteller:
EnkiLife
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
FC, ICC, IHC, WB
Spezies Reaktivität:
Human, Mouse, Rat
Konjugation:
Unconjugated
Alternative Synonym:
protein kinase C beta type,PRKCB,Prkcb,PKC-B, PKC-beta,PRKCB1,PKC Beta-I,EC:2.7.11.13
Produktbeschreibung
Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Calcium-activated, phospholipid- and diacylglycerol (DAG)- dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR- induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at Ser-559, Ser-644 and Ser-652. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at Ser-180, which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity (PubMed:11598012). Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates Ser-36 of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and specifically mediating phosphorylation of Thr-6 of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 Lys-4 (H3K4me) by LSD1/KDM1A (PubMed: 20228790). In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. Participates in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Phosphorylates SLC2A1/GLUT1, promoting glucose uptake by SLC2A1/GLUT1 (PubMed: 25982116). Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription (PubMed: 19176525).
Produkteigenschaften
Klonalität:
Monoclonal
Molekulargewicht:
77kDa
NCBI:
5579
UniProt:
P05771
Puffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Reinheit:
Affinity purification
Formulierung:
Liquid
Target-Kategorie:
PRKCB
Anwendungsbeschreibung
Application Verdünnung:
WB 1:500-1:2000,IHC 1:50-1:200,ICC/IF 1:100-1:200,FC 1:100-1:200
