NUP98 antibody [2H10]

Artikelnummer: GTX-GTX00697
Artikelname: NUP98 antibody [2H10]
Artikelnummer: GTX-GTX00697
Hersteller Artikelnummer: GTX00697
Alternativnummer: GTX00697-100
Hersteller: Genetex
Wirt: Rat
Kategorie: Antikörper
Applikation: DOT, ELISA, ICC, IF, WB
Spezies Reaktivität: Human, Mouse, Rat, Yeast
Immunogen: Recombinant GST-fused human Nup98 (amino acids 1-466)
Konjugation: Unconjugated
Alternative Synonym: ADIR2, GLFG-repeat containing nucleoporin, nuclear pore complex protein Nup98, nuclear pore complex protein Nup98-Nup96, nucleoporin 98kD, nucleoporin 98kDa, NUP196, NUP96, NUP98/PHF23 fusion 2 protein, Nup98-Nup96
Klonalität: Monoclonal
Konzentration: 1 mg/ml
Klon-Bezeichnung: 2H10
Isotyp: IgG2c
NCBI: 4928
UniProt: P52948
Puffer: Filter-sterilized PBS, 50% glycerol
Reinheit: Purified IgG
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: nucleoporin 98
Anwendungsbeschreibung: WB: Assay dependent. ICC/IF: Assay dependent. Dot: Assay dependent. ELISA: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
ICC/IF analysis of HeLa cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The signal at the nuclear periphery with 21A10 mAb was much higher and the background lower than that of 2H10 and 13C2 antibodies.
Green : Primary antibody
Violet : DAPI
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min
ICC/IF analysis of S. pombe cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min
WB analysis of S. pombe cell extracts expressing wilde type Nup98 (Lane 1) or Nup98-GFP protein (Lane 2) using GTX00697 NUP98 antibody [2H10].
Dilution : 1 μg/ml
WB analysis of HeLa nuclear membrane extract using GTX00697 NUP98 antibody [2H10].
Dilution : 1:2000
ICC/IF analysis of HeLa cells using GTX00697 NUP98 antibody [2H10].
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min
Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications.
WB analysis of S. cereviciae cell extracts using GTX00697 NUP98 antibody [2H10].
Five protein bands identified by this antibody correspond to the sizes of 5 kinds of nucleoporins with multiple GFLG motifs in S. pombe.
Dilution : 2 μg/ml
ICC/IF analysis of S. cereviciae cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min
ICC/IF analysis of rat neuron cells using GTX00697 NUP98 antibody [2H10].
The dots are Nup98.