ELAVL1 / HuR antibody [GT1182], IgG, Unconjugated, Rabbit, Monoclonal

Artikelnummer: GTX-GTX00944
Artikelname: ELAVL1 / HuR antibody [GT1182], IgG, Unconjugated, Rabbit, Monoclonal
Artikelnummer: GTX-GTX00944
Hersteller Artikelnummer: GTX00944
Alternativnummer: GTX00944-100
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC-P, WB, IF, ICC
Spezies Reaktivität: Rat, Human, Mouse
Immunogen: A synthesized peptide derived from human HuR / ELAVL1.
Konjugation: Unconjugated
Alternative Synonym: ELAV like RNA binding protein 1 , ELAV1 , ELAVL1 , HUR , Hua , MelG , HuR , HuA
Klonalität: Monoclonal
Konzentration: Batch dependent (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: GT1182
Molekulargewicht: 36
Isotyp: IgG
NCBI: 1994
UniProt: Q15717
Puffer: PBS (pH 7.3), 0.02% sodium azide, 50% glycerol
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: ELAV like RNA binding protein 1
Application Verdünnung: WB: 1:500-1:2000. ICC/IF: 1:50-1:200. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Anwendungsbeschreibung: WB: 1:500-1:2000. ICC/IF: 1:50-1:200. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ELAVL1 / HuR antibody [GT1182] (GTX00944) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
WB analysis of wild-type (WT) and ELAVL1 / HuR knockout (KO) 293T cell extracts using GTX00944 ELAVL1 / HuR antibody [GT1182].
Dilution : 1:1000
Laoding : 25μg
WB analysis of various samples using GTX00944 ELAVL1 / HuR antibody [GT1182].
Dilution : 1:1000
Loading : 25μg
IHC-P analysis of mouse spleen tissue section using GTX00944 ELAVL1 / HuR antibody [GT1182].
Dilution : 1:100
ICC/IF analysis of PFA-fixed C6 cells using GTX00944 ELAVL1 / HuR antibody [GT1182].
Orange : Primary antibody
Blue : DAPI
ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00944 ELAVL1 / HuR antibody [GT1182].
Orange : Primary antibody
Blue : DAPI
ICC/IF analysis of PFA-fixed U2OS cells using GTX00944 ELAVL1 / HuR antibody [GT1182].
Orange : Primary antibody
Blue : DAPI
IHC-P analysis of rat liver tissue section using GTX00944 ELAVL1 / HuR antibody [GT1182].
Dilution : 1:100
IHC-P analysis of human esophageal tissue section using GTX00944 ELAVL1 / HuR antibody [GT1182].
Dilution : 1:100