LC3B antibody [GT1187]

Artikelnummer: GTX-GTX00949
Artikelname: LC3B antibody [GT1187]
Artikelnummer: GTX-GTX00949
Hersteller Artikelnummer: GTX00949
Alternativnummer: GTX00949-100
Hersteller: Genetex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IF, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: A synthetic peptide of human LC3B.
Konjugation: Unconjugated
Alternative Synonym: ATG8F, Autophagy-related protein LC3 B, autophagy-related ubiquitin-like modifier LC3 B, LC3B, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1LC3B-a, Microtubule-associated protein 1 light chain 3 beta, microtubule-associated proteins 1A/1B light chain 3B
Klonalität: Monoclonal
Konzentration: Batch dependent
Klon-Bezeichnung: GT1187
Isotyp: IgG
NCBI: 81631
UniProt: Q9GZQ8
Puffer: PBS (pH 7.3), 0.02% sodium azide, 50% glycerol
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: microtubule associated protein 1 light chain 3 beta
Anwendungsbeschreibung: WB: 1:500-1:2000. ICC/IF: 1:50-1:200. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT1187] (GTX00949) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
IHC-P analysis of Rat brain tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100
IHC-P analysis of Mouse spinal cord tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100
ICC/IF analysis of PFA-fixed C6 cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated C6 cells
Left : C6 cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100
ICC/IF analysis of PFA-fixed HeLa cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated HeLa cells
Left : HeLa cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100
IHC-P analysis of Human brain tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100
ICC/IF analysis of PFA-fixed NIH-3T3 cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated NIH-3T3 cells
Left : NIH-3T3 cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100
WB analysis of wild-type (WT) and LC3B knockout (KO) 293T cell extracts with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Dilution : 1:1000
Loading : 25μg
WB analysis of C6 and NIH-3T3 cell extracts with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Dilution : 1:1000
Loading : 25μg