LC3B antibody [GT1187], IgG, Unconjugated, Rabbit, Monoclonal

Produktübersicht

• Artikelname: LC3B antibody [GT1187], IgG, Unconjugated, Rabbit, Monoclonal
• Artikelnummer: GTX-GTX00949
• Hersteller Artikelnummer: GTX00949
• Alternativnummer: GTX00949-100
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IHC-P, WB, IF, ICC
• Spezies Reaktivität: Rat, Human, Mouse
• Immunogen: A synthetic peptide of human LC3B.
• Konjugation: Unconjugated
• Alternative Synonym: microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B , MAP1LC3Ba , APG8b

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: Batch dependent (Please refer to the vial label for the specific concentration.)
• Klon-Bezeichnung: GT1187
• Molekulargewicht: 15
• Isotyp: IgG
• NCBI: 81631
• UniProt: Q9GZQ8
• Puffer: PBS (pH 7.3), 0.02% sodium azide, 50% glycerol
• Lagerung: 2°C to 8°C, -20°C or -80°C
• Target-Kategorie: microtubule associated protein 1 light chain 3 beta

Anwendungsbeschreibung

• Application Verdünnung: WB: 1:500-1:2000. ICC/IF: 1:50-1:200. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
• Anwendungsbeschreibung: WB: 1:500-1:2000. ICC/IF: 1:50-1:200. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT1187] (GTX00949) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

IHC-P analysis of Rat brain tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100

IHC-P analysis of Mouse spinal cord tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100

ICC/IF analysis of PFA-fixed C6 cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated C6 cells
Left : C6 cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

ICC/IF analysis of PFA-fixed HeLa cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated HeLa cells
Left : HeLa cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

IHC-P analysis of Human brain tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100

ICC/IF analysis of PFA-fixed NIH-3T3 cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated NIH-3T3 cells
Left : NIH-3T3 cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

WB analysis of wild-type (WT) and LC3B knockout (KO) 293T cell extracts with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Dilution : 1:1000
Loading : 25μg

WB analysis of C6 and NIH-3T3 cell extracts with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Dilution : 1:1000
Loading : 25μg