MLKL (phospho Ser358) antibody

Artikelnummer: GTX-GTX00973
Artikelname: MLKL (phospho Ser358) antibody
Artikelnummer: GTX-GTX00973
Hersteller Artikelnummer: GTX00973
Alternativnummer: GTX00973-100
Hersteller: Genetex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: A synthesized peptide derived from human MLKL around the phosphorylation site of Ser358.
Konjugation: Unconjugated
Alternative Synonym: hMLKL, mixed lineage kinase domain-like protein
Klonalität: Polyclonal
Konzentration: 1 mg/ml
Isotyp: IgG
NCBI: 197259
UniProt: Q8NB16
Puffer: PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol
Reinheit: Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: mixed lineage kinase domain like pseudokinase
Anwendungsbeschreibung: WB: 1:500-1:2000. IHC-P: 1:50-1:200. ELISA: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
IHC-P analysis of rat heart tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00973 MLKL (phospho Ser358) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml
IHC-P analysis of mouse muscle tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
IHC-P analysis of human gastric cancer tissue sections using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
WB analysis of HeLa cell lysate using GTX00973 MLKL (phospho Ser358) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
IHC-P analysis of mouse heart tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
IHC-P analysis of mouse heart tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
IHC-P analysis of rat heart tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
IHC-P analysis of rat muscle tissue using GTX00973 MLKL (phospho Ser358) antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100