Iba1 antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX101495
Artikelname: Iba1 antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX101495
Hersteller Artikelnummer: GTX101495
Alternativnummer: GTX101495-25,GTX101495-100
Hersteller: Genetex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB, ICC/IF, IHC-P, FACS, IP, IHC
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: AIF-1, allograft inflammatory factor 1, IBA1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, IRT1, IRT-1, protein G1
Klonalität: Polyclonal
Konzentration: 0.56 mg/ml (Please refer to the vial label for the specific concentration.)
Isotyp: IgG
NCBI: 199
UniProt: P55008
Puffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: allograft inflammatory factor 1
Anwendungsbeschreibung: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:500. IHC: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
 Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 Iba1 antibody detects Iba1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody (GTX101495) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.
 Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody (GTX101495) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.
 Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
 Iba1 antibody detects Iba1 protein at microglia in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 antibody (GTX101495) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 Immunoprecipitation of Iba1 protein from K562 whole cell extracts using 5 μg of Iba1 antibody (GTX101495).
Western blot analysis was performed using Iba1 antibody (GTX101495).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
 Flow cytometry on primary murine microglia cells, staining with Iba1 (GTX101495) antibody using 1.0 μg per 4×105 cells. GTX101495 (blue), Rabbit IgG (green) ,Unstained (red).

 Iba1 antibody (GTX101495) detects AIF1 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: Iba1 antibody (GTX101495) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.