Iba1 antibody

Artikelnummer: GTX-GTX101495
Artikelname: Iba1 antibody
Artikelnummer: GTX-GTX101495
Hersteller Artikelnummer: GTX101495
Hersteller: Genetex
Wirt: Rabbit
Applikation: WB, IF, ICC, IHC-P, IHC-Fr, FACS, IP, IHC
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary.
Konjugation: IgG
Alternative Synonym: Allograft Inflammatory Factor 1,Aif-1,Iba1,Irt-1,Irt1,Aif1
Klonalität: Polyclonal
Konzentration: 0.56 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 17
Isotyp: IgG
NCBI: 199
UniProt: P55008
Puffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Formulierung: Liquid
Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4-8ºC. For long-term storage, aliquot and store at -20-8ºC or below. Avoid multiple freeze-thaw cycles.
Anwendungsbeschreibung: WB: 1:500-1:10000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications., ICC/IF: 1:100-1:1000.*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other...
 Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Iba1 protein stained by Iba1 antibody (GTX101495) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
 Immunoprecipitation of Iba1 protein from K562 whole cell extracts using 5 μg of Iba1 antibody (GTX101495).
Western blot analysis was performed using Iba1 antibody (GTX101495).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
 Iba1 antibody detects Iba1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Flow cytometry on primary murine microglia cells, staining with Iba1 (GTX101495) antibody using 1.0 μg per 4×105 cells. GTX101495 (blue), Rabbit IgG (green) ,Unstained (red).

 Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
 Iba1 antibody detects Iba1 protein at microglia in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Iba1 antibody (GTX101495) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 Iba1 antibody (GTX101495) detects AIF1 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: Iba1 antibody (GTX101495) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
 Iba1 antibody detects Iba1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 Iba1 antibody detects Iba1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.