MURF1 antibody [N1], N-term

Artikelnummer: GTX-GTX110475
Artikelname: MURF1 antibody [N1], N-term
Artikelnummer: GTX-GTX110475
Hersteller Artikelnummer: GTX110475
Alternativnummer: GTX110475-25,GTX110475-100
Hersteller: Genetex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IF, IHC-P, WB
Spezies Reaktivität: Human, Mammal, Mouse, Porcine, Rat, Xenopus
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MURF1. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: E3 ubiquitin-protein ligase TRIM63, IRF, iris ring finger protein, MURF1, MURF2, muscle specific ring finger protein 2, muscle-specific RING finger protein 1, ring finger protein 28, RING-type E3 ubiquitin transferase TRIM63, RNF28, SMRZ, striated muscle RING zinc finger protein, tripartite motif containing 63, E3 ubiquitin protein ligase, tripartite motif-containing protein 63
Klonalität: Polyclonal
Konzentration: 2.25 mg/ml
Isotyp: IgG
NCBI: 84676
UniProt: Q969Q1
Puffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: tripartite motif containing 63
Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody [N1], N-term (GTX110475) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded human breast cancer, using MURF1(GTX110475) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
MURF1 antibody detects MURF1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody (GTX110475) diluted by 1:1000.
MURF1 antibody [N1], N-term detects MURF1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MURF1 protein stained by MURF1 antibody [N1], N-term (GTX110475) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody [N1], N-term (GTX110475) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody [N1], N-term (GTX110475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.