ECHS1 antibody [N1C2], IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX114376
Artikelname: ECHS1 antibody [N1C2], IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX114376
Hersteller Artikelnummer: GTX114376
Alternativnummer: GTX114376-25,GTX114376-100
Hersteller: Genetex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC-P, WB, IP, ICC
Spezies Reaktivität: Mouse, Human
Immunogen: Recombinant protein encompassing a sequence within the center region of human ECHS1. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: enoyl-CoA hydratase, short chain 1 , ECHS1D , SCEH
Klonalität: Polyclonal
Konzentration: 1.01 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 31
Isotyp: IgG
NCBI: 1892
UniProt: P30084
Puffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: enoyl-CoA hydratase, short chain 1
Application Verdünnung: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Anwendungsbeschreibung: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Immunoprecipitation of ECHS1 protein from 293T whole cell extracts using 5 μg of ECHS1 antibody [N1C2] (GTX114376).
Western blot analysis was performed using ECHS1 antibody [N1C2] (GTX114376).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ECHS1 protein stained by ECHS1 antibody [N1C2] (GTX114376) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114376 diluted at 1:1000
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
ECHS1 antibody detects ECHS1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 antibody (GTX114376) diluted by 1:5000.
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.
Green: ECHS1 protein stained by ECHS1 antibody [N1C2] (GTX114376) diluted at 1:200.
Blue: Hoechst 33342 staining.
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.
_x000D_
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.
_x000D_
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min