DNMT1 antibody [N1], N-term, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX116011
Artikelname: DNMT1 antibody [N1], N-term, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX116011
Hersteller Artikelnummer: GTX116011
Alternativnummer: GTX116011-25,GTX116011-100
Hersteller: GeneTex International
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IP, ICC, IHC-P, WB, ChIP
Spezies Reaktivität: Human
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human DNMT1. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: DNA methyltransferase 1 , ADCADN , AIM , CXXC9 , DNMT , HSN1E , MCMT , m.HsaI
Klonalität: Polyclonal
Konzentration: 0.18 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 183
Isotyp: IgG
NCBI: 1786
UniProt: P26358
Puffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: DNA methyltransferase 1
Application Verdünnung: WB: 1:1000-1:10000. ICC/IF: 1:100-1:2000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. ChIP assay: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Anwendungsbeschreibung: WB: 1:1000-1:10000. ICC/IF: 1:100-1:2000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. ChIP assay: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNMT1 protein stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:2000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Wild-type (WT) and DNMT1 knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Cross-linked ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or anti-DNMT1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ILIR2.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
DNMT1 antibody [N1], N-term immunoprecipitates DNMT1 protein in IP experiments.
IP samples: HeLa nuclear extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of DNMT1 protein by 4 μg DNMT1 antibody [N1], N-term (GTX116011)
5 % SDS-PAGE
The immunoprecipitated DNMT1 protein was detected by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
PMID: 22455563"> The IHC-P analysis of DNMT1 antibody [N1] was published by Xu X and colleagues in the journal Diagn Pathol in 2012.PMID: 22455563
PMID: 22455563"> The IHC-P analysis of DNMT1 antibody [N1] was published by Xu X and colleagues in the journal Diagn Pathol in 2012.PMID: 22455563