TSG101 antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX118736
Artikelname: TSG101 antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX118736
Hersteller Artikelnummer: GTX118736
Alternativnummer: GTX118736-25,GTX118736-100
Hersteller: GeneTex International
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IP, ICC, IHC-P, WB, ELISA
Spezies Reaktivität: Mouse, Human
Immunogen: Recombinant protein encompassing a sequence within the center region of human TSG101. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: tumor susceptibility 101 , TSG10 , VPS23
Klonalität: Polyclonal
Konzentration: 0.87 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 44
Isotyp: IgG
NCBI: 7251
UniProt: Q99816
Puffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: tumor susceptibility 101
Application Verdünnung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. ELISA: 1:1000-1:10000. Sandwich ELISA: Assay dependent. Capture: GTX118736 , Detection: GTX70255. *Optimal dilutions/concentrations should be determined by the researcher.Not
Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. ELISA: 1:1000-1:10000. Sandwich ELISA: Assay dependent. Capture: GTX118736 , Detection: GTX70255. *Optimal dilutions/concentrations should be determined by the researcher.Not t
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody (GTX118736) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TSG101 antibody detects TSG101 protein by Western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
10 % SDS-PAGE
TSG101 antibody (GTX118736) dilution: 1:500
TSG101 antibody detects TSG101 protein at cytoplasm by immunofluorescent analysis.
Sample: A549 cells were fixed in 2% paraformaldehyde at RT for 15 min, and be permeabilized in 0.1% saponin, 10% NGS, 100mM glycine for 30 min.
Green: TSG101 protein stained by TSG101 antibody (GTX118736) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunoprecipitation of TSG101 protein from A549 whole cell extracts using 5 μg of TSG101 antibody (GTX118736).
Western blot analysis was performed using TSG101 antibody (GTX118736) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody (GTX118736) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody (GTX118736) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ELISA detection of TSG101 using GTX118736 for capture at a concentration of 5 μg/mL and GTX70255 for detection at a concentration of 1.5 μg/mL.
TSG101 antibody detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
TSG101 stained by TSG101 antibody (GTX118736) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody (GTX118736) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.