Paxillin antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX125891
Artikelname: Paxillin antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX125891
Hersteller Artikelnummer: GTX125891
Alternativnummer: GTX125891-25,GTX125891-100
Hersteller: GeneTex International
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC-P, WB, IP, ICC
Spezies Reaktivität: Mouse, Human, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human Paxillin. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: paxillin
Klonalität: Polyclonal
Konzentration: 1.77 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 65
Isotyp: IgG
NCBI: 5829
UniProt: P49023
Puffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: paxillin
Application Verdünnung: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Anwendungsbeschreibung: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Paxillin antibody detects Paxillin protein by western blot analysis.
A. 1 μg 293T whole cell extract
B. 1 μg whole cell extract of GFP-human Paxillin-transfected 293T cells
7.5% SDS-PAGE
Paxillin antibody (GTX125891) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Paxillin antibody detects Paxillin protein at cytoskeleton by immunofluorescent analysis.
Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin protein stained by Paxillin antibody (GTX125891) diluted at 1:100.
Red: phalloidin staining.
Courtesy of a researcher who prefers to be anonymous.
Paxillin antibody immunoprecipitates Paxillin protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 2 μg of preimmune Rabbit IgG
B. Immunoprecipitation of Paxillin protein by 2 μg Paxillin antibody (GTX125891)
7.5 % SDS-PAGE
The immunoprecipitated Paxillin protein was detected by Paxillin antibody (GTX125891) diluted at 1:10000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Wild-type (WT) and Paxillin knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Paxillin antibody detects Paxillin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Paxillin stained by Paxillin antibody (GTX125891) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Paxillin antibody detects Paxillin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Paxillin stained by Paxillin antibody (GTX125891) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min