Paxillin antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelname:	Paxillin antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	GTX-GTX125891
Hersteller Artikelnummer:	GTX125891
Alternativnummer:	GTX125891-25,GTX125891-100
Hersteller:	GeneTex International
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IHC-P, WB, IP, ICC
Spezies Reaktivität:	Mouse, Human, Rat
Immunogen:	Recombinant protein encompassing a sequence within the center region of human Paxillin. The exact sequence is proprietary.
Konjugation:	Unconjugated
Alternative Synonym:	paxillin

Klonalität:	Polyclonal
Konzentration:	1.77 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht:	65
Isotyp:	IgG
NCBI:	5829
UniProt:	P49023
Puffer:	1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit:	Purified by antigen-affinity chromatography.
Lagerung:	2°C to 8°C, -20°C or -80°C
Target-Kategorie:	paxillin

Application Verdünnung:	WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Anwendungsbeschreibung:	WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

	Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Paxillin antibody detects Paxillin protein by western blot analysis. A. 1 μg 293T whole cell extract B. 1 μg whole cell extract of GFP-human Paxillin-transfected 293T cells 7.5% SDS-PAGE Paxillin antibody (GTX125891) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Paxillin antibody detects Paxillin protein at cytoskeleton by immunofluorescent analysis. Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Paxillin protein stained by Paxillin antibody (GTX125891) diluted at 1:100. Red: phalloidin staining. Courtesy of a researcher who prefers to be anonymous.
	Paxillin antibody immunoprecipitates Paxillin protein in IP experiments. IP samples: HeLa whole cell extract A. Control with 2 μg of preimmune Rabbit IgG B. Immunoprecipitation of Paxillin protein by 2 μg Paxillin antibody (GTX125891) 7.5 % SDS-PAGE The immunoprecipitated Paxillin protein was detected by Paxillin antibody (GTX125891) diluted at 1:10000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
	Wild-type (WT) and Paxillin knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Paxillin antibody detects Paxillin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Paxillin stained by Paxillin antibody (GTX125891) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
	Paxillin antibody detects Paxillin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Paxillin stained by Paxillin antibody (GTX125891) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min