SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag, Unconjugated

Artikelnummer: GTX-GTX135592-PRO
Artikelname: SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag, Unconjugated
Artikelnummer: GTX-GTX135592-PRO
Hersteller Artikelnummer: GTX135592-pro
Alternativnummer: GTX135592-PRO-100
Hersteller: GeneTex
Kategorie: Proteine/Peptide
Applikation: WB, Sandwich ELISA, ELISA
Spezies Reaktivität: SARS Coronavirus 2
Konjugation: Unconjugated
Alternative Synonym: 2019-nCoV Nucleocapsid, 2019-nCoV NP, COVID-19, SARS-CoV-2 Nucleocapsid, SARS-CoV-2 NP, COVID-19 NP, SARS-COV-2, COV2, NCOV, COVID, CORONA, COVID-19 Nucleocapsid, COV-2, SARS-COV, COVID19
Konzentration: Batch dependent (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 46
Tag: HIS Tag
Puffer: PBS
Lagerung: -20°C
Application Verdünnung: Recommended antibody pairs for sandwich ELISA:Capture: GTX635685, Detection: GTX635712/GTX635688/GTX635678.Please notice that GTX635688/GTX635678 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685. Pleas
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL.
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.
Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) and (GTX135592-pro) at concentrations ranging from 0.015 μg/mL to 1 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135361) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
0.5 μg of GTX135592-pro SARS-CoV-2 (COVID-19) nucleocapsid protein analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.