Trident femto Western HRP Substrate

Artikelnummer: GTX-GTX14698
Artikelname: Trident femto Western HRP Substrate
Artikelnummer: GTX-GTX14698
Hersteller Artikelnummer: GTX14698
Hersteller: Genetex
Kategorie: Biochemikalien
Applikation: WB
Spezies Reaktivität: Human
Alternative Synonym: Trident femto-ECL
Formulierung: Liquid
Lagerung: Trident femto Western HRP Substrate is stable for 18 months at 4-86,. Keep container tightly closed and store Trident femto Western HRP Substrate away from heat or light. Mixed substrate can be stored for up to 24 hours at 2-8-8ºC.
Anwendungsbeschreibung: please refer to GTX14698 Protocol
 10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.
 Recombinant protein mcherry is loaded as the follows: Lane A 3.13 ng recombinant protein Lane B 1.56 ng recombinant protein Lane C 780 pg recombinant protein Lane D 390 pg recombinant protein The blot was probed with mcherry antibody (GTX128508) diluted at 1:2000. A HRP-conjugated secondary antibody was applied and developed with 1 ml of Trident femto Western HRP Substrate (GTX14698). Exposure time : 18 seconds, 54 seconds, and 90 seconds. SuperSignalTM is a registered trademark of Thermo Fisher Scientific. The trademark holder is not affiliated with GeneTex and does not endorse this product.
 Serial dilutions of purified mCherry recombinant protein were prepared and applied at 10 μL/well. After electrophoresis and protein transfer, membranes were probed with anti-mCherry antibody (GTX128508) diluted at 1:1500 and then with goat anti-rabbit HRP-conjugated secondary antibody (1:2000). Identical blots were incubated with 1 ml of Trident femto Western HRP Substrate, SuperSignalTM West Femto, Trident pico Western HRP Substrate, ClarityTM, and LuminataTM Forte. The blots were simultaneously exposed for 90 seconds and 180 seconds using BioSpectrumTM 500 digital imaging system SuperSignalTM West Femto, ClarityTM, LuminataTM Forte are registered trademark of Thermo Fisher Scientific, Bio-Rad, and EMD Millipore, respectively. The trademark holder is not affiliated with GeneTex and does not endorse this product.
 Various 293T whole cell extracts were prepared and applied at 10 μL/well. After electrophoresis and protein transfer, membranes were probed with alpha Tubulin antibody (GTX112141) diluted at 1:100000 and then with goat anti-rabbit HRP-conjugated secondary antibody (GTX213110-01). Identical blots were incubated with 1 ml of Trident femto Western HRP Substrate and the mixed substrate (Substrate solution A and Substrate solution B) stored for 0, 15 and 24 hours at 2-8°C. The blots were simultaneously exposed for 18 seconds, 90 seconds and 180 seconds using BioSpectrumTM 500 digital imaging system. The signal was developed with Trident femto Western HRP Substrate (GTX14698).