Human alpha Synuclein protein (active, Pre-Formed Fibrils)

Artikelnummer: GTX-GTX17669-PRO
Artikelname: Human alpha Synuclein protein (active, Pre-Formed Fibrils)
Artikelnummer: GTX-GTX17669-PRO
Hersteller Artikelnummer: GTX17669-PRO
Alternativnummer: GTX17669-PRO-100,GTX17669-PRO-200
Hersteller: Genetex
Kategorie: Proteine/Peptide
Applikation: Functional Assay
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: alpha-synuclein, I+/--synuclein, NACP, non A-beta component of AD amyloid, PARK1, PARK4, PD1, synuclein alpha-140, synuclein, alpha (non A4 component of amyloid precursor), truncated alpha synuclein
Konzentration: Batch dependent
NCBI: 6622
Puffer: PBS
Reinheit: Purified by ion-exchange chromatography
Lagerung: -20°C
Target-Kategorie: synuclein alpha
Anwendungsbeschreibung: Endogenous alpha-synuclein phosphorylation. 100 µM alpha synuclein protein monomer (GTX17668-pro) seeded with 10 nM alpha synuclein protein PFF (GTX17669-pro) in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated a fluorescen
SDS-PAGE of ~14 kDa active Human Recombinant Alpha Synuclein Protein Preformed Fibrils (GTX17669-pro). Lane 1: Molecular Weight Ladder (MW). Lane 2: active Alpha Synuclein Protein Preformed Fibrils (GTX17669-pro).
Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Preformed Fibrils (GTX17669-pro) at 4 μg/ml (D-F), but not when treated with control Alpha Synuclein Protein Preformed Fibrils (GTX17667-pro) at 4 μg/ml (A-C).
Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4ºC. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body inclusions. Magnification: 20x.
Immunohistochemistry analysis of rat brain injected with active human alpha synuclein PFFs (GTX17669-pro). Species: Female Sprague-Dawley Rat. Rat was injected with 2μL active human alpha synuclein PFFs (GTX17669-pro) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30-days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the striatum close to an injection site.
Active alpha synuclein preformed fibrils (GTX17669-pro) seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers (GTX17668-pro). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 nM of active alpha synuclein preformed fibrils (GTX17669-pro) is combined with 100 μM of alpha synuclein monomer (GTX17668-pro), as compared to active alpha synuclein preformed fibrils (GTX17669-pro) alone and alpha synuclein monomer (GTX17668-pro) alone. Thioflavin T ex = 450 nm, em = 485 nm.
TEM of active human alpha synuclein preformed fibrils (GTX17669-pro). Fibrils were sonicated and treated with uranyl acetate. Top: 50kx magnification. Bottom: 100kx magnification.
TEM of active human alpha synuclein preformed fibrils (GTX17669-pro) (top) and control human alpha synuclein preformed fibrils (GTX17667-pro) (bottom). Fibrils were sonicated and treated with uranyl acetate. The active fibrils are shorter than the control fibrils.
TEM of active human alpha synuclein preformed fibrils (GTX17669-pro) (top) and control human alpha synuclein preformed fibrils (GTX17667-pro) (bottom). Fibrils were sonicated and treated with uranyl acetate. The active fibrils are shorter than the control fibrils.