easy iPSC

Artikelnummer: GTX-GTX400001
Artikelname: easy iPSC
Artikelnummer: GTX-GTX400001
Hersteller Artikelnummer: GTX400001
Hersteller: Genetex
Kategorie: Zellen/Zellkultur
Applikation: ICC, IF, PCR
Spezies Reaktivität: Human, Mouse
Alternative Synonym: minicircle DNA, easy-iPSC, easyiPSC, iPSC Minicircle, easy iPSC
Konzentration: 1 mg/ml
Puffer: 0.1X TE Buffer
Formulierung: Liquid
Lagerung: -20°C
Anwendungsbeschreibung: This protocol has been optimized for use in human adipose stem cells (hASC). Use in other cell types, such as fibroblasts, will require optimization by the researcher.
 easy iPSC induces the expression of endogenous OCT4 in hASC as determined by RT-PCR. Primers specific for the 3' UTR of endogenous OCT4 (GTX80102) were used for analysis. Image from Jia et al. 2010. Nature Methods, 7(3).
 easy iPSC induces the expression of endogenous OCT4 in hASC as determined by RT-PCR. Primers specific for the 3' UTR of endogenous OCT4 (GTX80102) were used for analysis. Image from Jia et al. 2010. Nature Methods, 7(3).
 10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.
 10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.
 10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.
 hASC transfected with easy iPSC express pluripotency markers. Image from Jia et al. 2010. Nature Methods, 7(3).
 10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.
 10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.
 RT-PCR analysis of markers for pluripotency and each germ layer confirms that easy iPSC-transfected hASC can be induced to differentiate into cells of each germ layer. Image from Jia et al. 2010. Nature Methods, 7(3).