This product contains 1% SDS, which is higher than what is in some other commercial RIPA lysis buffers, and should facilitate a more thorough extraction of nuclear protein. The sample may be viscous following extraction due to unsheared DNA. To decrease t
A431 and HeLa cells were lysed by using GTX400005 RIPA lysis buffer. Whole cell extracts (30 μg) were assayed by Western Blot analysis. The membrane was blotted with a cytosolic protein GAPDH antibody (GTX627408), a membrane protein Flotillin1 antibody (GTX104769) and a nuclear protein LaminB2 (GTX628803).
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