Trident RIPA Lysis Buffer

Artikelnummer: GTX-GTX400005
Artikelname: Trident RIPA Lysis Buffer
Artikelnummer: GTX-GTX400005
Hersteller Artikelnummer: GTX400005
Alternativnummer: GTX400005-100
Hersteller: Genetex
Kategorie: Biochemikalien
Applikation: WB
Lagerung: 2°C to 8°C
Anwendungsbeschreibung: This product contains 1% SDS, which is higher than what is in some other commercial RIPA lysis buffers, and should facilitate a more thorough extraction of nuclear protein. The sample may be viscous following extraction due to unsheared DNA. To decrease t
A431 and HeLa cells were lysed by using GTX400005 RIPA lysis buffer.
Whole cell extracts (30 μg) were assayed by Western Blot analysis.
The membrane was blotted with a cytosolic protein GAPDH antibody (GTX627408), a membrane protein Flotillin1 antibody (GTX104769) and a nuclear protein LaminB2 (GTX628803).