TTC32 antibody [1F7], IgG1, Unconjugated, Mouse, Monoclonal

Artikelnummer: GTX-GTX83486
Artikelname: TTC32 antibody [1F7], IgG1, Unconjugated, Mouse, Monoclonal
Artikelnummer: GTX-GTX83486
Hersteller Artikelnummer: GTX83486
Alternativnummer: GTX83486-100
Hersteller: GeneTex
Wirt: Mouse
Kategorie: Antikörper
Applikation: IHC-P, WB, IF, ICC, FACS
Spezies Reaktivität: Human
Immunogen: Full length human recombinant protein of human TTC32(NP_001008238) produced in HEK293T cell.
Konjugation: Unconjugated
Alternative Synonym: tetratricopeptide repeat domain 32
Klonalität: Monoclonal
Konzentration: 0.63 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: 1F7
Molekulargewicht: 17
Isotyp: IgG1
NCBI: 130502
UniProt: Q5I0X7
Puffer: PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Reinheit: Purified by affinity chromatography
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: tetratricopeptide repeat domain 32
Application Verdünnung: WB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Anwendungsbeschreibung: WB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
FACS analysis of HEK293T cells transfected with either TTC32 plasmid(Red) or empty vector control plasmid(Blue) using GTX83486 TTC32 antibody [1F7].
ICC/IF analysis of COS7 cells transiently transfected with TTC32 plasmid using GTX83486 TTC32 antibody [1F7].
WB analysis of HEK293T cells transfected with TTC32 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83486 TTC32 antibody [1F7].
Loading : 5 ug per lane
IHC-P analysis of human colon adenocarcinoma tissue using GTX83486 TTC32 antibody [1F7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
IHC-P analysis of human pancreas tissue using GTX83486 TTC32 antibody [1F7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
IHC-P analysis of human prostate tissue using GTX83486 TTC32 antibody [1F7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
IHC-P analysis of human kidney tissue using GTX83486 TTC32 antibody [1F7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
FACS analysis of Jurkat cells using GTX83486 TTC32 antibody [1F7].
Red : Primary antibody
Blue : Negative control antibody
IHC-P analysis of human ovary adenocarcinoma tissue using GTX83486 TTC32 antibody [1F7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50