DCK antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: DCK antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX102800
• Hersteller Artikelnummer: GTX102800
• Alternativnummer: GTX102800-100,GTX102800-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC, IHC-P, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human DCK. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: deoxycytidine kinase

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.41 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 31
• NCBI: 1633
• UniProt: P27707
• Puffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:1000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX102800 IHC-P Image

DCK antibody detects DCK protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12% SDS-PAGE
DCK antibody (GTX102800) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX102800) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DCK antibody detects DCK protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
DCK stained by DCK antibody (GTX102800) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX102800) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DCK antibody detects DCK protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DCK protein stained by DCK antibody (GTX102800) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

DCK antibody detects DCK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX102800) diluted at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of DCK protein from 293T whole cell extracts using 5 μg of DCK antibody (GTX102800).
Western blot analysis was performed using DCK antibody (GTX102800).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX102800) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.