RUVBL1 antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: RUVBL1 antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX111294
• Hersteller Artikelnummer: GTX111294
• Alternativnummer: GTX111294-100,GTX111294-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC-P, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RUVBL1. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: RuvB like AAA ATPase 1 , ECP-54 , ECP54 , INO80H , NMP 238 , NMP238 , PONTIN , Pontin52 , RVB1 , TIH1 , TIP49 , TIP49A

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.32 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 50
• NCBI: 8607
• UniProt: Q9Y265
• Puffer: 1XPBS (pH7), 1% BSA, 20% Glycerol, 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX111294 IP Image

RUVBL1 antibody detects RUVBL1 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RUVBL1 protein stained by RUVBL1 antibody (GTX111294) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RUVBL1(GTX111294) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RUVBL1 antibody detects RUVBL1 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX111294) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX111294) diluted at 1:1000.