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GTX113029 WB Image |
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Sample (30 μg of whole cell lysate) A: A549 B: HeLa C: HepG2 7.5% SDS PAGE GTX113029 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
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TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRAF6 protein stained by TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:100. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.
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TRAF6 antibody [N2C2], Internal immunoprecipitates TRAF6 protein in IP experiments. IP samples: K562 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of TRAF6 protein by 4 μg TRAF6 antibody [N2C2], Internal (GTX113029) 7.5 % SDS-PAGE The immunoprecipitated TRAF6 protein was detected by TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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TRAF6 antibody [N2C2], Internal detects TRAF6 protein by western blot analysis. A. 30 μg PC-12 whole cell extract B. 30 μg Rat2 whole cell extract 7.5% SDS-PAGE TRAF6 antibody [N2C2], Internal (GTX113029) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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Immunohistochemical analysis of paraffin-embedded 59T xenograft, using TRAF6(GTX113029) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
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TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
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Sample (30 μg of whole cell lysate) A: NIH-3T3 B: BCL-1 7.5% SDS PAGE GTX113029 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |