ROCK1 antibody [N1N2], N-term, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: ROCK1 antibody [N1N2], N-term, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX113266
• Hersteller Artikelnummer: GTX113266
• Alternativnummer: GTX113266-100,GTX113266-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC-P, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.35 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 158
• NCBI: 6093
• UniProt: Q13464
• Puffer: 1XPBS pH7, 1% BSA, 20% Glycerol, 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX113266 IHC-P Image

ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
5% SDS-PAGE
ROCK1 antibody [N1N2], N-term (GTX113266) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell extract
B. 30 μg C2Cl2 whole cell extract
5% SDS-PAGE
ROCK1 antibody [N1N2], N-term (GTX113266) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ROCK1 antibody immunoprecipitates ROCK1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of ROCK1 by 3 μg of ROCK1 antibody (GTX113266) 5% SDS-PAGE The immunoprecipitated ROCK1 protein was detected by ROCK1 antibody (GTX113266) diluted at 1 : 500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ROCK1 antibody [N1N2], N-term detects ROCK1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ROCK1 protein stained by ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Wild-type (WT) and ROCK1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.