TDP43 antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: TDP43 antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX114210
• Hersteller Artikelnummer: GTX114210
• Alternativnummer: GTX114210-100,GTX114210-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ChIP, ICC, IHC-P, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: TAR DNA binding protein , ALS10 , TDP-43

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.63 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 45
• NCBI: 23435
• UniProt: Q13148
• Puffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX114210 ICC/IF Image

TDP43 antibody detects TDP43 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TDP43 stained by TDP43 antibody (GTX114210) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using TDP-43(GTX114210) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

TARDBP antibody immunoprecipitates TARDBP protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TARDBP protein by 2 μg of TARDBP antibody (GTX114210) 12% SDS-PAGE The immunoprecipitated TARDBP protein was detected by TARDBP antibody (GTX114210) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

TARDBP antibody detects TARDBP protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg BCL-1 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
10% SDS-PAGE
TARDBP antibody (GTX114210) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GTX114210) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Western blot analysis of TDP-43 (GTX114210, upper panel) and beta-actin (GTX109639, lower panel)?
Sample (30 μg of whole cell lysate)
? A: HeLa mock control?
B: HeLa transfected shTDP-43
10% SDS PAGE?
GTX114210 diluted at 1:1000.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

TDP43 antibody detects TDP43 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TDP43 stained by TDP43 antibody (GTX114210) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

TDP43 antibody detects TDP43 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TDP43 stained by TDP43 antibody (GTX114210) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GTX114210) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.