SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active), Unconjugated

Artikelnummer: GTX135817-PRO
Artikelname: SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active), Unconjugated
Artikelnummer: GTX135817-PRO
Hersteller Artikelnummer: GTX135817-pro
Alternativnummer: GTX135817-PRO-100
Hersteller: GeneTex
Kategorie: Proteine/Peptide
Applikation: ELISA, Receptor Binding Assays, WB
Spezies Reaktivität: Virus
Konjugation: Unconjugated
Alternative Synonym: COVID-19 Spike, SARS-CoV-2 Spike, covid-19, sars-cov-2
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active), Unconjugated
Konzentration: 0.97 mg/ml (Please refer to the vial label for the specific concentration.)
Puffer: PBS, no Preservative.
Expression System: HEK293 cells
Formulierung: Liquid
Sequenz: SARS-CoV-2 Spike of QHD43416.1 (1-685 a.a) with His tag at the C-terminus
Anwendungsbeschreibung: Recommended antibody pair for sandwich ELISA: Capture: GTX635807, Detection: GTX635792-01 / GTX635793-01/ GTX635791.Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please c
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX135817-pro) (4000-62.5 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody (GTX135384) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (3000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
1 μg of GTX135817-pro SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX135817-pro, 1 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike antibody (GTX135356) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX135817-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with the SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.
1 μg of GTX135817-pro SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.
Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 (L18F..., R190S, K417T, E484K, N501Y, D614G, H655Y), His tag (active) (Brazil variant) (GTX136094-pro), SARS-CoV-2 (COVID-19) Spike S1 (L18F, D80A,..., R246I, K417N, E484K, N501Y, D614G), His tag (active) (South Africa variant) (GTX136095-pro) and SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13.72-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (14-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.
GTX135817-pro ELISA Image
Sandwich ELISA detection of recombinant Spike S1 proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) using antibodies as below._x000D__x000D_
Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) _x000D_
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL)._x000D_

Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.
Sandwich ELISA detection of recombinant Spike S1 proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) using antibodies as below._x000D__x000D_
Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) _x000D_
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL).