easy iPSC

Produktübersicht

• Artikelname: easy iPSC
• Artikelnummer: GTX400001
• Hersteller Artikelnummer: GTX400001
• Alternativnummer: GTX400001-100
• Hersteller: GeneTex
• Kategorie: Sonstiges
• Applikation: ICC, Cell Culture, RT-PCR
• Spezies Reaktivität: Human, Mouse
• Alternative Synonym: minicircle DNA , easy-iPSC , easyiPSC , iPSC Minicircle , easy iPSC

Produktbeschreibung

Minicircle DNA for iPSC Production. Easy iPSC is a simple non-viral solution for reprogramming terminally differentiated cells into pluripotent stem cells. One simple transfection delivers all four transcription factors required. This specially designed

Produkteigenschaften

• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Puffer: 0.1X TE Buffer, no Preservative.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: This protocol has been optimized for use in human adipose stem cells (hASC). Use in other cell types, such as fibroblasts, will require optimization by the researcher.

Bilder

RT-PCR analysis of markers for pluripotency and each germ layer confirms that easy iPSC-transfected hASC can be induced to differentiate into cells of each germ layer. Image from Jia et al. 2010. Nature Methods, 7(3).

easy iPSC induces the expression of endogenous OCT4 in hASC as determined by RT-PCR. Primers specific for the 3' UTR of endogenous OCT4 (GTX80102) were used for analysis. Image from Jia et al. 2010. Nature Methods, 7(3).

hASC transfected with easy iPSC express pluripotency markers. Image from Jia et al. 2010. Nature Methods, 7(3).