SARS-CoV Spike overexpression 293T whole cell lysate

Artikelnummer: GTX535668
Artikelname: SARS-CoV Spike overexpression 293T whole cell lysate
Artikelnummer: GTX535668
Hersteller Artikelnummer: GTX535668
Alternativnummer: GTX535668-200
Hersteller: GeneTex
Kategorie: Proteine/Peptide
Applikation: WB
Spezies Reaktivität: Virus
Alternative Synonym: sars-cov
Whole-cell lysate of 293T cells overexpressing SARS-CoV spike protein (full-length, no tag). The translated amino acid sequence is based on AAP13567.1. Lysate prepared in RIPA buffer with no reducing agents and is unboiled. Please see matched control 293
Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Puffer: 50 mM Tris-HCl (pH8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 µg/ml Leupeptin, 2 µg/ml Aprotinin, 1 µg/ml Pepstatin A, 0.025% ProClin 300.
Formulierung: Liquid
GTX535668 WB Image
Non-transfected and transfected 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV Spike (SΔ3) antibody [7G12] (GTX632603) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.