Histone H3K9me1 (mono-methyl Lys9) antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX54101
Artikelname: Histone H3K9me1 (mono-methyl Lys9) antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX54101
Hersteller Artikelnummer: GTX54101
Alternativnummer: GTX54101-100
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ChIP, DOT, ICC, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
Konjugation: Unconjugated
Alternative Synonym: histone cluster 3 H3 | H3.4 | H3/g | H3FT | H3t
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core h
Klonalität: Polyclonal
Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Polyclonal
Molekulargewicht: 16
Isotyp: IgG
NCBI: 8290
UniProt: Q16695
Puffer: PBS pH7.3, 50% Glycerol, 0.02% Sodium azide.
Quelle: Human
Reinheit: Purified by affinity chromatography
Formulierung: Liquid
Anwendungsbeschreibung: WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. ChIP assay: 1:20 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Dot blot analysis of all sorts of methylation peptides using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody.
ChIP analysis of 293 cell lysate using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
ICC/IF analysis of 293T cells using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody.
Blue : DAPI
ICC/IF analysis of C6 cells using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody.
Blue : DAPI
Dilution : 1:100
WB analysis of various sample lysates using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody.
Dilution : 1:1000
Loading : 25μg per lane
IHC-P analysis of human embryo brain tissue using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody.
Dilution : 1:200
ICC/IF analysis of HeLa cells using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody.
Blue : DAPI
Dilution : 1:100
ICC/IF analysis of NIH/3T3 cells using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody.
Blue : DAPI
Dilution : 1:100
GTX54101 IHC-P Image