alpha Smooth Muscle Actin antibody [GT445], IgG1, Clone: Monoclonal, Unconjugated, Human

Artikelnummer: GTX629702
Artikelname: alpha Smooth Muscle Actin antibody [GT445], IgG1, Clone: Monoclonal, Unconjugated, Human
Artikelnummer: GTX629702
Hersteller Artikelnummer: GTX629702
Alternativnummer: GTX629702-100,GTX629702-25
Hersteller: GeneTex
Wirt: Human
Kategorie: Antikörper
Applikation: WB, IHC-P, ICC
Spezies Reaktivität: Human, Mouse, Rat, Drosophila
Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Smooth Muscle Actin. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: actin alpha 2, smooth muscle , ACTSA
Klonalität: Monoclonal
Konzentration: 0.75 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Monoclonal
Molekulargewicht: 42
Isotyp: IgG1
NCBI: 59
UniProt: P62736
Puffer: PBS
Reinheit: Affinity purified by Protein G.
alpha Smooth Muscle Actin antibody [GT445] detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha Smooth Muscle Actin antibody [GT445] detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha smooth muscle Actin antibody [GT445] detects alpha smooth muscle Actin protein by western blot analysis.
A. 30 μg Rat-2 whole cell lysate/extract
10% SDS-PAGE
alpha smooth muscle Actin antibody [GT445] (GTX629702) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
alpha smooth muscle Actin antibody [GT445] detects alpha smooth muscle Actin protein by western blot analysis.
A. 30 μg drosophila lysate/extract
10% SDS-PAGE
alpha smooth muscle Actin antibody [GT445] (GTX629702) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
alpha smooth muscle Actin antibody [GT445] detects alpha smooth muscle Actin protein by western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
D. 30 μg Raw264.7 whole cell lysate/extract
E. 30 μg C2C12 whole cell lysate/extract
10% SDS-PAGE
alpha smooth muscle Actin antibody [GT445] (GTX629702) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
alpha Smooth Muscle Actin antibody [GT445] detects alpha Smooth Muscle Actin protein at cytoplasm and cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Smooth Muscle Actin protein stained by alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:500.
Blue: Hoechst 33342 staining.
alpha Smooth Muscle Actin antibody [GT445] detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human breast cancer.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, 20 min