TDP43 antibody [GT225], IgG2b, Clone: Monoclonal, Unconjugated, Human

Artikelnummer: GTX630196
Artikelname: TDP43 antibody [GT225], IgG2b, Clone: Monoclonal, Unconjugated, Human
Artikelnummer: GTX630196
Hersteller Artikelnummer: GTX630196
Alternativnummer: GTX630196-100,GTX630196-25
Hersteller: GeneTex
Wirt: Human
Kategorie: Antikörper
Applikation: WB, IHC-P, ICC
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: TAR DNA binding protein , ALS10 , TDP-43
Klonalität: Monoclonal
Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Monoclonal
Molekulargewicht: 45
Isotyp: IgG2b
NCBI: 23435
UniProt: Q13148
Puffer: PBS
Reinheit: Affinity purified by Protein G.
TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:1000
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TARDBP antibody [GT225] (GTX630196) diluted at 1:500.
TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
B. 30 μg Raw264.7 whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:1000
TDP43 antibody [GT225] detects TDP43 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TDP43 antibody [GT225] (GTX630196) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TDP43 antibody [GT225] detects TDP43 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TDP43 antibody [GT225] (GTX630196) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TARDBP antibody [GT225] validation by siRNA knock-down.
Upperpanel: TARDBP antibody [GT225] GTX630196
Lower panel: GAPDH antibody (GTX100118)
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of TARDBP shRNA-transfected 293T cells
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:3000
GAPDH antibody (GTX100118) dilution: 1:10000
TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:1000
TDP43 antibody [GT225] detects TDP43 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nestin stained by Nestin antibody (GTX133112) diluted at 1:500.
Red: TDP43 stained by TDP43 antibody [GT225] (GTX630196) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
TDP43 antibody [GT225] detects TDP43 protein immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nestin stained by Nestin antibody (GTX133111) diluted at 1:500.
Red: TDP43 stained by TDP43 antibody [GT225] (GTX630196) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).