NDP52 antibody [GT422], IgG1, Clone: Monoclonal, Unconjugated, Mouse

Artikelnummer: GTX630396
Artikelname: NDP52 antibody [GT422], IgG1, Clone: Monoclonal, Unconjugated, Mouse
Artikelnummer: GTX630396
Hersteller Artikelnummer: GTX630396
Alternativnummer: GTX630396-25,GTX630396-100
Hersteller: GeneTex
Wirt: Mouse
Kategorie: Antikörper
Applikation: ICC, IP, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: calcium binding and coiled-coil domain 2 , NDP52
Klonalität: Monoclonal
Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Monoclonal
Molekulargewicht: 52
Isotyp: IgG1
NCBI: 10241
UniProt: Q13137
Puffer: PBS
Reinheit: Affinity purified by Protein G.
NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX630396) diluted by 1:500.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.
NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis.
Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 16 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with 100% MeOH for 30 sec.
Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 μg of NDP52 antibody [GT422] (GTX630396).
Western blot analysis was performed using NDP52 antibody [GT422] (GTX630396).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX630396) diluted by 1:500.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
NDP52 antibody [GT422] detects NDP52 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
NDP52 antibody [GT422] (GTX630396) dilution: 1:1000
NDP52 antibody [GT422] detects NDP52 protein by western blot analysis.
A. 30 μg Huh7 whole cell lysate/extract
B. 30 μg Hep3B whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
NDP52 antibody [GT422] (GTX630396) dilution: 1:1000
NDP52 antibody [GT422] detects NDP52 protein at autophagosome? by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NDP52 stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.