Arginase 1 antibody [GT5811], IgG2a, Clone: Monoclonal, Unconjugated, Mouse

Artikelnummer: GTX634218
Artikelname: Arginase 1 antibody [GT5811], IgG2a, Clone: Monoclonal, Unconjugated, Mouse
Artikelnummer: GTX634218
Hersteller Artikelnummer: GTX634218
Alternativnummer: GTX634218-25,GTX634218-100
Hersteller: GeneTex
Wirt: Mouse
Kategorie: Antikörper
Applikation: IHC-P, ELISA, FACS, WB
Spezies Reaktivität: Mouse, Human, Rat
Immunogen: Full length human Arginase 1 recombinant protein.
Konjugation: Unconjugated
Alternative Synonym: arginase 1
Klonalität: Monoclonal
Konzentration: 3.26 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Monoclonal
Molekulargewicht: 35
Isotyp: IgG2a
NCBI: 383
UniProt: P05089
Puffer: PBS
Reinheit: Affinity purified by Protein A.
Arginase 1 antibody [GT5811] (GTX634218) detects Arginase 1 protein by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: Arginase 1 antibody [GT5811] (GTX634218) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
Whole cell extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX109242 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX113131 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX109242 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Arginase 1 antibody [GT5811] detects Arginase 1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min