Profilin 1 antibody [2D2], IgG1, Unconjugated, Mouse, Monoclonal

Artikelnummer: GTX83902
Artikelname: Profilin 1 antibody [2D2], IgG1, Unconjugated, Mouse, Monoclonal
Artikelnummer: GTX83902
Hersteller Artikelnummer: GTX83902
Alternativnummer: GTX83902-100
Hersteller: GeneTex
Wirt: Mouse
Kategorie: Antikörper
Applikation: FACS, ICC, IHC-P, WB
Spezies Reaktivität: Canine, Human, Monkey, Rat
Immunogen: Full length human recombinant protein of human PFN1 (NP_005013) produced in HEK293T cell.
Konjugation: Unconjugated
Alternative Synonym: profilin 1 , ALS18
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
Klonalität: Monoclonal
Konzentration: 0.58 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: [2D2]
Molekulargewicht: 15
Isotyp: IgG1
NCBI: 5216
UniProt: P07737
Puffer: PBS pH7.3, 1% BSA, 50% Glycerol, 0.02% Sodium azide.
Quelle: Human
Reinheit: Purified by affinity chromatography
Formulierung: Liquid
Anwendungsbeschreibung: WB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
IHC-P analysis of human pancreas tissue using GTX83902 Profilin 1 antibody [2D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
WB analysis of various cell lines using GTX83902 Profilin 1 antibody [2D2].
Loading : 35 ug per lane
FACS analysis of Jurkat cells using GTX83902 Profilin 1 antibody [2D2].
Red : Primary antibody
Blue : Negative control antibody
IHC-P analysis of human kidney tissue using GTX83902 Profilin 1 antibody [2D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
IHC-P analysis of human prostate tissue using GTX83902 Profilin 1 antibody [2D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
FACS analysis of HeLa cells using GTX83902 Profilin 1 antibody [2D2].
Red : Primary antibody
Blue : Negative control antibody
GTX83902 FACS Image
WB analysis of HEK293T cells transfected with Profilin 1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83902 Profilin 1 antibody [2D2].
Loading : 5 ug per lane
ICC/IF analysis of COS7 cells transiently transfected with Profilin 1 plasmid using GTX83902 Profilin 1 antibody [2D2].