Neurogenin 1 antibody [9A8], IgG1, Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: Neurogenin 1 antibody [9A8], IgG1, Unconjugated, Mouse, Monoclonal
• Artikelnummer: GTX84051
• Hersteller Artikelnummer: GTX84051
• Alternativnummer: GTX84051-100
• Hersteller: GeneTex
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ICC, IHC-P, IP, WB
• Spezies Reaktivität: Human
• Immunogen: Full length human recombinant protein of human NEUROG1 (NP_006152) produced in HEK293T cell.
• Konjugation: Unconjugated
• Alternative Synonym: neurogenin 1 , AKA , Math4C , NEUROD3 , bHLHa6 , ngn1

Produktbeschreibung

Appears to mediate neuronal differentiation.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 0.75 mg/ml (Please refer to the vial label for the specific concentration.)
• Klon-Bezeichnung: [9A8]
• Molekulargewicht: 26
• Isotyp: IgG1
• NCBI: 4762
• UniProt: Q92886
• Puffer: PBS pH7.3, 1% BSA, 50% Glycerol, 0.02% Sodium azide.
• Quelle: Human
• Reinheit: Purified by affinity chromatography
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. IP: 2-4ug/mg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

ICC/IF analysis of COS7 cells transiently transfected with Neurogenin 1 plasmid using GTX84051 Neurogenin 1 antibody [9A8].

IP analysis of DDDDK tagged Neurogenin 1 overexpressed HEK293T lysate using GTX84051 Neurogenin 1 antibody [9A8].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

WB analysis of HEK293T cells transfected with Neurogenin 1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84051 Neurogenin 1 antibody [9A8].
Loading : 5 ug per lane

IHC-P analysis of human prostate tissue using GTX84051 Neurogenin 1 antibody [9A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human kidney tissue using GTX84051 Neurogenin 1 antibody [9A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human lung tissue using GTX84051 Neurogenin 1 antibody [9A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

WB analysis of various cell lines using GTX84051 Neurogenin 1 antibody [9A8].
Loading : 35 ug per lane

GTX84051 IHC-P Image

IHC-P analysis of human breast tissue using GTX84051 Neurogenin 1 antibody [9A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.