LTA4H antibody [9C5], IgG2b, Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: LTA4H antibody [9C5], IgG2b, Unconjugated, Mouse, Monoclonal
• Artikelnummer: GTX84176
• Hersteller Artikelnummer: GTX84176
• Alternativnummer: GTX84176-100
• Hersteller: GeneTex
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: IHC-P, IP, WB
• Spezies Reaktivität: Canine, Human, Monkey, Mouse
• Immunogen: Full-length protein expressed in 293T cell transfected with human LTA4H expression vector
• Konjugation: Unconjugated
• Alternative Synonym: leukotriene A4 hydrolase

Produktbeschreibung

Hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). The enzyme also has some peptidase activity.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Klon-Bezeichnung: [9C5]
• Molekulargewicht: 69
• Isotyp: IgG2b
• NCBI: 4048
• UniProt: P09960
• Puffer: PBS pH7.3, 1% BSA, 50% Glycerol, 0.02% Sodium azide.
• Quelle: Human
• Reinheit: Purified by affinity chromatography
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:200. IHC-P: 1:100-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

WB analysis of various cell lines using GTX84176 LTA4H antibody [9C5].
Loading : 10 ug per lane
Dilution : 1:200

GTX84176 WB Image

IHC-P analysis of pancreas tissue using GTX84176 LTA4H antibody [9C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

IHC-P analysis of endometrium tissue using GTX84176 LTA4H antibody [9C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

IHC-P analysis of bladder carcinoma tissue using GTX84176 LTA4H antibody [9C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

IHC-P analysis of colon tissue using GTX84176 LTA4H antibody [9C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

IHC-P analysis of prostate tissue using GTX84176 LTA4H antibody [9C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

IP analysis of DDDDK tagged LTA4H overexpressed HEK293T lysate using GTX84176 LTA4H antibody [9C5].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

WB analysis of HEK293T cells transfected with LTA4H plasmid (Right) or empty vector (Left) for 48 hrs using GTX84176 LTA4H antibody [9C5].
Loading : 5 ug per lane