alpha B Crystallin antibody [6D11], IgG1, Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: alpha B Crystallin antibody [6D11], IgG1, Unconjugated, Mouse, Monoclonal
• Artikelnummer: GTX84657
• Hersteller Artikelnummer: GTX84657
• Alternativnummer: GTX84657-100
• Hersteller: GeneTex
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FACS, ICC, IHC-P, IP, WB
• Spezies Reaktivität: Human, Monkey, Rat
• Immunogen: Full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.
• Konjugation: Unconjugated
• Alternative Synonym: crystallin alpha B , CMD1II , CRYA2 , CTPP2 , CTRCT16 , HEL-S-101 , HSPB5 , MFM2

Produktbeschreibung

May contribute to the transparency and refractive index of the lens.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Klon-Bezeichnung: [6D11]
• Molekulargewicht: 20
• Isotyp: IgG1
• NCBI: 1410
• UniProt: P02511
• Puffer: PBS pH7.3, 1% BSA, 50% Glycerol, 0.02% Sodium azide.
• Quelle: Human
• Reinheit: Purified by affinity chromatography
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500. ICC/IF: 1:50-1:100. IHC-P: 1:50. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

FACS analysis of HEK293T cells transfected with either alpha B Crystallin plasmid(Red) or empty vector control plasmid(Blue) using GTX84657 alpha B Crystallin antibody [6D11].

WB analysis of HEK293T cells transfected with alpha B Crystallin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84657 alpha B Crystallin antibody [6D11].
Loading : 5 ug per lane

ICC/IF analysis of COS7 cells transiently transfected with alpha B Crystallin plasmid using GTX84657 alpha B Crystallin antibody [6D11].

IP analysis of DDDDK tagged alpha B Crystallin overexpressed HEK293T lysate using GTX84657 alpha B Crystallin antibody [6D11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84657 alpha B Crystallin antibody [6D11].
Loading : 10 ug per lane
Dilution : 1:200

IHC-P analysis of human thyroid carcinoma tissue using GTX84657 alpha B Crystallin antibody [6D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84657 IHC-P Image

WB analysis of various cell lines using GTX84657 alpha B Crystallin antibody [6D11].
Loading : 35 ug per lane

ICC/IF analysis of HT29 cells using GTX84657 alpha B Crystallin antibody [6D11].