Rabbit IgG anti-Goat IgG (H+L)-ATTO 647N, MinX Ms,Rb, ATTO-647N, Polyclonal

Produktübersicht

• Artikelname: Rabbit IgG anti-Goat IgG (H+L)-ATTO 647N, MinX Ms,Rb, ATTO-647N, Polyclonal
• Artikelnummer: ROC-605-456-013
• Hersteller Artikelnummer: 605-456-013
• Alternativnummer: ROC-605-456-013
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FLISA,IF,WB
• Spezies Reaktivität: Goat
• Immunogen: Goat IgG whole molecule
• Konjugation: ATTO 647N
• Alternative Synonym: rabbit anti-Goat IgG ATTO 647N Conjugated Antibody, rabbit anti-Goat IgG Antibody ATTO647N Conjugation
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): Mouse,Rabbit

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Goat
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: >1:10,000
• Anwendungsbeschreibung: Anti-Goat IgG (H&L) conjugated by ATTO 647N has been tested by dot blot and western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is a

Bilder

Dot Blot Results of Rabbit Anti-Goat IgG (H&L) Antibody (MX Hu, Ms, Rb) ATTO 647N Conjugated. Sample: Goat IgG [p/n 005-0102]. Loaded: 1) 100ng, 2) 33.33ng, 3) 11.11ng, 4) 3.7ng, 5) 1.23ng. Antibody: Anti-Goat IgG (H&L) [Rabbit] Antibody (MX3) ATTO 647N Conjugated at 1.0µg/mL for 1hr at RT. Block: Blocking Buffer for Fluorescent Western Blotting [p/n MB-070] for 30 mins at RT.

ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH

Comparison of nephrin imaging with different conventional and super-resolution modalities. A) Widefield vs dSTORM using Atto647N. B) Confocal vs STED using Atto647N. C) Widefield vs SIM using Alexa Fluor 488. D) ExM prepared sample imaged with widefield vs e confocal using Alexa Fluor 488. The confocal ExM image furthermore shows the nucleus stained with Hoechst. Highlighted panels show *3 zoomed areas. Scale bars in a-c are 1µm, in d and e 10µm and in the zoomed in areas 5µm (after expansion) Figure provided by CiteAb. Source: Anal Bioanal Chem, PMID: 33277998.