Rabbit IgG (H&L) Antibody DyLight(TM) 488 Conjugated Pre-Adsorbed, DL488, Sheep, Polyclonal

Produktübersicht

• Artikelname: Rabbit IgG (H&L) Antibody DyLight(TM) 488 Conjugated Pre-Adsorbed, DL488, Sheep, Polyclonal
• Artikelnummer: ROC-611-641-122
• Hersteller Artikelnummer: 611-641-122
• Alternativnummer: ROC-611-641-122
• Hersteller: Rockland Immunochemicals
• Wirt: Sheep
• Kategorie: Antikörper
• Spezies Reaktivität: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Konjugation: DL488
• Alternative Synonym: Sheep Anti Rabbit IgG Antibody DyLight 488(TM) Conjugate, Sheep Anti-Rabbit IgG DyLight 488(TM) Conjugated Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Rabbit
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: >1:10,000
• Anwendungsbeschreibung: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor

Bilder

Properties of DyLight(TM) Fluorescent Dyes.

DyLight(TM) dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. DyLight(TM) dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods. This image shows anti-histone detection using a DyLight(TM) 488 conjugate (green). Anti-Tubulin was detected using a DyLight(TM) 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).

DyLight(TM) 488 Fluorescence Spectra.

Immunohistochemistry. NOS3 increases 3-nitrotyrosine content in heart tissue in developing sepsis. Immunohistochemical staining demonstrated an increase of nitrotyrosine in heart tissue of septic wildtype mice at 6 hours post CLP, whereas no differences could be detected in NOS3-/- mice. Heart samples were fixed in 4% formaldehyde phosphate buffer, dehydrated and paraffin-embedded. For 3-nitrotyrosin detection serial sections (3 per mouse, 400µm apart) were incubated with a polyclonal antibody raised against nitrotyrosine, followed by sheep anti-rabbit IgG conjugated to DyLight(TM)488 (p/n 611-641-122).Figure online resource 1. PMID: 23397596.