AGAROSE LENS CULINARIS AGGLUTININ 10 ml

Artikelnummer: VEC-AL-1043-10
Artikelname: AGAROSE LENS CULINARIS AGGLUTININ 10 ml
Artikelnummer: VEC-AL-1043-10
Hersteller Artikelnummer: AL-1043-10
Alternativnummer: VEC-AL-1043-10
Hersteller: Vector Laboratories
Kategorie: Biochemikalien
Konjugation: Agarose

Features:

  • Matrix is heat stable, cross-linked 4% agarose beads with a molecular exlusion of about 2x107 daltons
  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Hydrophilic spacer arm is inserted between the lectin and the matrix
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation.  This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer
  • 3 mg lectin/ml gel
  • Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside or Glycoprotein Eluting Solution (ES-1100)
    Technische Daten und Beschreibung  
       
       
    Unit Size 10 ml
    Applications Glycobiology, Affinity Chromatography
    Recommended Storage 2-8 °C DO NOT FREEZE
    Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.08% sodium azide, 10 mM mannose, 0.01 mM MnCl2
    Recommended Usage Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-1100. Alternatively, 200mM α−methyl mannoside/200mM α−methyl glucoside mixture can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
    Matrix Conjugate Lectins
    Sugar Specificity Mannose, Glucose
    Conjugate Agarose