Tubulin beta-III Recombinant Rabbit Monoclonal Antibody, Clone: [3B7], Unconjugated

Product Overview

• Article Name: Tubulin beta-III Recombinant Rabbit Monoclonal Antibody, Clone: [3B7], Unconjugated
• Biozol Catalog Number: BYT-ORB612226
• Supplier Catalog Number: orb612226
• Alternative Catalog Number: BYT-ORB612226-100,BYT-ORB612226-25,BYT-ORB612226-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthesized peptide derived from human beta III Tubulin (400-450/450aa)
• Conjugation: Unconjugated
• Alternative Names: Neuron specific beta III Tubulin, beta 4, MC1R, TBB3_HUMAN, TUBB 3, TUBB 4, TUBB3, TUBB4, Tubulin beta 3 chain, Tubulin beta 4, Tubulin beta III, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, beta tubulin III, Neuron specific beta III Tubulin.

Product Description

Tubulin beta-III Recombinant Rabbit Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1mg/ml
• Clone Designation: [3B7]
• Molecular Weight: 50 kDa
• UniProt: Q13509
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: TUBB3

Application Notes

• Application Dilute: WB=1:1000-10000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1:50-200

Images

Blank control: N2A. Primary Antibody (green line): Rabbit Anti-TUBB3 antibody (orb612226), dilution: 1:50, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Tubulin beta-III) Monoclonal Antibody, Unconjugated (orb612226) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human left parietal lobe), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Tubulin beta-III) Monoclonal Antibody, Unconjugated (orb612226) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Tubulin beta-III) Monoclonal Antibody, Unconjugated (orb612226) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Tubulin beta-III) Monoclonal Antibody, Unconjugated (orb612226) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Tubulin beta-III) Monoclonal Antibody, Unconjugated (orb612226) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Rat Cerebellum tissue lysates, Lane 5: Human SH-SY5Y cell lysates, Lane 6: Human U251 cell lysates, Lane 7: Human U87MG cell lysates, Primary: Anti-Tubulin beta-III (orb612226) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kDa, Observed band size: 50 kDa.

SHG-44 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Tubulin beta-III (3B7)) Monoclonal Antibody, Unconjugated (orb612226) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Tubulin beta-III (3B7)) Monoclonal Antibody, Unconjugated (orb612226) 1:50, 90 minutes