Dengue virus NS3 protein antibody, IgG, Unconjugated, Rabbit, Polyclonal

Article Name:	Dengue virus NS3 protein antibody, IgG, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	GTX-GTX124252
Supplier Catalog Number:	GTX124252
Alternative Catalog Number:	GTX124252-100,GTX124252-25
Manufacturer:	GeneTex
Host:	Rabbit
Category:	Antikörper
Application:	IHC-P, IF, ICC, IHC, WB, FACS
Species Reactivity:	Dengue virus
Immunogen:	Recombinant protein encompassing a sequence within the center region of Dengue virus NS3 protein. The exact sequence is proprietary.
Conjugation:	Unconjugated
Alternative Names:	Dengue Virus Non-structural protein 3 , Dengue Virus NS3 , DENV Non-structural protein 3 , DENV NS3

Clonality:	Polyclonal
Concentration:	1.3 mg/ml (Please refer to the vial label for the specific concentration.)
Molecular Weight:	70
Isotype:	IgG
Buffer:	1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Purity:	Purified by antigen-affinity chromatography.
Storage:	2°C to 8°C, -20°C or -80°C
Target:	Nonstructural protein NS3

Application Dilute:	WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. IHC-P: Assay dependent. FACS: Assay dependent. IHC: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Application Notes:	WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. IHC-P: Assay dependent. FACS: Assay dependent. IHC: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

PMID: 29559699">	The IHC-P analysis of Dengue virus NS3 protein antibody was published by Sarathy VV and colleagues in the journal Sci Rep in 2018.PMID: 29559699
	NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000. Blue: Hoechst 33342 staining.
	NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000. Blue: Hoechst 33342 staining.
	Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS3 (Dengue virus 2)(GTX124252) antibody at 1:2000 dilution.
	NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000. Blue: Hoechst 33342 staining.
	Sample (20 μg of whole cell lysate) A: BHK-21 B: Dengue virus 2 infect BHK-21 C: JEV infect BHK-21 7.5% SDS PAGE GTX124252 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis. A. 30 μg BHK-21 whole cell extract B. 30 μg whole cell extract of Dengue virus type 1 infected BHK-21 cells C. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells D. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells E. 30 μg whole cell extract of Dengue virus type 4 infected BHK-21 cells 7.5% SDS-PAGE NS3 (Dengue virus) antibody (GTX124252) dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:2000.
	NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000. Blue: Hoechst 33342 staining.