USP7 antibody, IgG, Unconjugated, Rabbit, Polyclonal

Catalog Number: GTX-GTX125894
Article Name: USP7 antibody, IgG, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: GTX-GTX125894
Supplier Catalog Number: GTX125894
Alternative Catalog Number: GTX125894-100,GTX125894-25
Manufacturer: GeneTex
Host: Rabbit
Category: Antikörper
Application: IHC-P, WB, IF, ICC, IP
Species Reactivity: Rat, Human, Mouse
Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human USP7. The exact sequence is proprietary.
Conjugation: Unconjugated
Alternative Names: ubiquitin specific peptidase 7 , HAUSP , TEF1
Clonality: Polyclonal
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Molecular Weight: 128
Isotype: IgG
NCBI: 7874
UniProt: Q93009
Buffer: 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purity: Purified by antigen-affinity chromatography.
Storage: 2°C to 8°C, -20°C or -80°C
Target: ubiquitin specific peptidase 7
Application Dilute: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
USP7 antibody detects USP7 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
USP7 antibody (GTX125894) dilution: 1:500.
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Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
5% SDS PAGE
GTX125894 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
USP7 antibody detects USP7 protein at cytosol and nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
USP7 antibody (GTX125894) dilution: 1:500.
_x000D_
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
5% SDS PAGE
GTX125894 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (30 μg of whole cell lysate)
A: PC-12
5% SDS PAGE
GTX125894 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
USP7 antibody detects USP7 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP7 protein stained by USP7 antibody (GTX125894) diluted at 1:500.
Blue: Hoechst 33343 staining.
USP7 antibody detects USP7 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
USP7 antibody (GTX125894) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunoprecipitation of USP7 protein from HepG2 whole cell extracts using 5 μg of USP7 antibody (GTX125894).
Western blot analysis was performed using USP7 antibody (GTX125894).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Sample (30 μg of whole cell lysate)
A: ?Non-transfected 293T lysates?
B: ?USP7 transfected 293T lysates
7.5% SDS PAGE
GTX125894 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.