beta Amyloid (1-42) antibody - Conformation Specific

Catalog Number: GTX-GTX134510
Article Name: beta Amyloid (1-42) antibody - Conformation Specific
Biozol Catalog Number: GTX-GTX134510
Supplier Catalog Number: GTX134510
Alternative Catalog Number: GTX134510-25,GTX134510-100
Manufacturer: Genetex
Host: Rabbit
Category: Antikörper
Application: DOT, ELISA, IHC-P
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Alternative Names: AAA, ABETA, ABPP, AD1, alzheimer disease amyloid protein, amyloid beta (A4) precursor protein, amyloid beta A4 protein, amyloid precursor protein, amyloid-beta A4 protein, Amyloid-beta precursor protein, APPI, beta-amyloid peptide, beta-amyloid peptide(1-40), beta-amyloid peptide(1-42), beta-amyloid precursor protein, cerebral vascular amyloid peptide, CTFgamma, CVAP, peptidase nexin-II, PN2, PN-II, preA4, protease nexin-II, testicular tissue protein Li 2
Clonality: Polyclonal
Concentration: 0.45 mg/ml
Isotype: IgG
NCBI: 351
UniProt: P05067
Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Purity: Purified by antigen-affinity chromatography.
Storage: 2°C to 8°C, -20°C or -80°C
Target: amyloid beta precursor protein
Application Notes: IHC-P: 1:100-1:1000. Dot: Assay dependent. ELISA: 1:1000-1:10000. sELISA: Assay dependent. Capture : GTX635160, Detection: GTX134510. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
 Sandwich ELISA detection of beta Amyloid (1-42)–peptide aggregate using GTX635160 as capture antibody at concentration of 10 μg/mL and GTX134510 as detection antibody at concentration of 0.45 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:2000 and used to detect the primary antibody.
 Beta amyloid (1-42) antibody (conformation specific) detects beta amyloid protein aggregates by immunohistochemical analysis in the hippocampus of 3xTg-AD transgenic mouse brain (left), but not in age- and gender- matched normal mouse brain (right).
Sample: Paraffin-embedded 3xTg-AD transgenic mouse brain (left) and healthy mouse brain (right).
beta Amyloid (1-42) stained by beta Amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0
 Conformation-specific beta amyloid antibody detects beta amyloid protein aggregates in the occipital lobe of human Alzheimer's disease brain by immunohistochemical analysis.
Antibodies: beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500, and competitor's antibody diluted at 1:500.

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 Beta amyloid (1-42) antibody (conformation specific) detects beta amyloid protein aggregates by immunohistochemical analysis in the occipital lobe of Alzheimer's disease brain (left), but not in normal human brain(right).
Sample: Paraffin-embedded Human Brain (Alzheimer's Disease + Normal) tissue slides (GTX49382).
beta amyloid protein aggregates stained by beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 ELISA plate is coated with 100 μL of beta Amyloid (1-42) peptide aggregate at 10 μg/mL. The coated protein is detected with beta Amyloid (1-42) antibody – Conformation Specific (GTX134510) at concentrations ranging from 45 ng/mL down to 1.67 ng/mL.
 Conformation-specific beta amyloid antibody detects beta amyloid protein aggregates in the postcentral gyrus of human Alzheimer's disease brain by immunohistochemical analysis.
Antibodies: beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500, and competitor's antibody diluted at 1:500.

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
 Dot blot analysis of 200ng beta amyloid peptide (1-42) oligomers or monomers, using Beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:4000, 1:8000, and 1:16000.