Endogenous alpha-synuclein phosphorylation. 100 µM alpha synuclein protein monomer (GTX17668-pro) seeded with 10 nM alpha synuclein protein PFF (GTX17669-pro) in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37C with shaking at 600 rpm. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
* VAT and and shipping costs not included. Errors and price changes excepted