easy iPSC

Catalog Number: GTX-GTX400001
Article Name: easy iPSC
Biozol Catalog Number: GTX-GTX400001
Supplier Catalog Number: GTX400001
Alternative Catalog Number: GTX400001-100
Manufacturer: GeneTex
Category: Kits/Assays
Application: Cell Culture, IF, ICC, RT-PCR
Species Reactivity: Human, Mouse
Alternative Names: minicircle DNA , easy-iPSC , easyiPSC , iPSC Minicircle , easy iPSC
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Buffer: 0.1X TE Buffer
Storage: -20°C
Application Dilute: This protocol has been optimized for use in human adipose stem cells (hASC). Use in other cell types, such as fibroblasts, will require optimization by the researcher.
Application Notes: This protocol has been optimized for use in human adipose stem cells (hASC). Use in other cell types, such as fibroblasts, will require optimization by the researcher.
easy iPSC induces the expression of endogenous OCT4 in hASC as determined by RT-PCR. Primers specific for the 3' UTR of endogenous OCT4 (GTX80102) were used for analysis. Image from Jia et al. 2010. Nature Methods, 7(3).
hASC transfected with easy iPSC express pluripotency markers. Image from Jia et al. 2010. Nature Methods, 7(3).
RT-PCR analysis of markers for pluripotency and each germ layer confirms that easy iPSC-transfected hASC can be induced to differentiate into cells of each germ layer. Image from Jia et al. 2010. Nature Methods, 7(3).