p53 antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: p53 antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX102965
• Supplier Catalog Number: GTX102965
• Alternative Catalog Number: GTX102965-100,GTX102965-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, ICC, IHC-P, IP, WB
• Species Reactivity: Human, Mouse, Zebrafish
• Immunogen: Recombinant protein encompassing a sequence within the center region of human p53. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Product Properties

• Clonality: Polyclonal
• Concentration: 1.28 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 44
• NCBI: 7157
• UniProt: P04637
• Buffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX102965 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using p53(GTX102965) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Non-transfected (–) and transfected (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX102965) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-p53 antibody (GTX102965).?(A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by GTX102965 diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit (GTX225856-01) was used in Western blot.

Sample (30 μg of whole cell lysate)
A: HCT116 cells with mock treatment for 24 hr
B: HCT116 cells with 30 μM cisplatin treatment for 24 hr
10% SDS PAGE
GTX102965 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

p53 antibody detects p53 protein at nucleus by immunofluorescent analysis.
Samples: HCT 116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 protein stained by p53 antibody (GTX102965) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

p53 antibody immunoprecipitates p53 protein-DNA complex in ChIP experiments. ChIP Sample: HCT116 whole cell lysate/extract treated with CPT 500nM for 6hr
A. 5 μg preimmune rabbit IgG
B. 5 μg of p53 antibody (GTX102965)
The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX102965) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

p53 antibody detects p53 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 stained by p53 antibody (GTX102965) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.