WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX110289 ICC/IF Image
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX110289 diluted at 1:1000
SAP102 antibody [C1C3] detects SAP102 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. SAP102 antibody [C1C3] (GTX110289) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Sample (30 ug of whole cell lysate) A: Molt-4 (GTX27912) 7.5% SDS PAGE GTX110298 diluted at 1:1000
SAP102 antibody [C1C3] detects SAP102 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SAP102 protein stained by SAP102 antibody [C1C3] (GTX110289) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining.
SAP102 antibody [C1C3] detects SAP102 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SAP102 protein stained by SAP102 antibody [C1C3] (GTX110289) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
SAP102 antibody [C1C3] detects SAP102 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. SAP102 antibody [C1C3] (GTX110289) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SAP102 antibody [C1C3] detects SAP102 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: SAP102 antibody [C1C3] stained by SAP102 antibody (GTX110289) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SAP102 antibody [C1C3] (GTX110289) diluted at 1:1000.
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