SAP102 antibody [C1C3], Unconjugated, Rabbit, Polyclonal

Catalog Number: GTX110289
Article Name: SAP102 antibody [C1C3], Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: GTX110289
Supplier Catalog Number: GTX110289
Alternative Catalog Number: GTX110289-100,GTX110289-25
Manufacturer: GeneTex
Host: Rabbit
Category: Antikörper
Application: ICC, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Porcine, Rat
Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human SAP102. The exact sequence is proprietary.
Conjugation: Unconjugated
Alternative Names: discs large MAGUK scaffold protein 3 , MRX , MRX90 , NEDLG , PPP1R82 , SAP102 , XLMR
Clonality: Polyclonal
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Molecular Weight: 90
NCBI: 1741
UniProt: Q92796
Buffer: 0.1M Tris-Glycine (pH7), 10% Glycerol, 0.01% Thimerosal.
Purity: Purified by antigen-affinity chromatography.
Form: Liquid
Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX110289 ICC/IF Image
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX110289 diluted at 1:1000
SAP102 antibody [C1C3] detects SAP102 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SAP102 antibody [C1C3] (GTX110289) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX110298 diluted at 1:1000
SAP102 antibody [C1C3] detects SAP102 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SAP102 protein stained by SAP102 antibody [C1C3] (GTX110289) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
SAP102 antibody [C1C3] detects SAP102 protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SAP102 protein stained by SAP102 antibody [C1C3] (GTX110289) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
SAP102 antibody [C1C3] detects SAP102 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SAP102 antibody [C1C3] (GTX110289) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SAP102 antibody [C1C3] detects SAP102 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: SAP102 antibody [C1C3] stained by SAP102 antibody (GTX110289) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SAP102 antibody [C1C3] (GTX110289) diluted at 1:1000.